Plasmid_Backbone

Part:BBa_K2259092:Design

Designed by: Laurynas Karpus   Group: iGEM17_Vilnius-Lithuania   (2017-10-27)


Minimal base vector for SynORI system building


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1349
    Illegal NheI site found at 177
    Illegal NheI site found at 1126
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 1355
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1349
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 1349
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 1349
    Plasmid lacks a suffix.
    Illegal XbaI site found at 1364
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

Minimal base vector for SynORI system building


Source

Minimal base vector for SynORI system building

References